102 Maintenance and Troubleshooting
7
Troubleshooting the Protein Application
Symptoms (Protein)
Broad Variability of the Lower Marker
Back to “Symptoms (Protein)” on page 89
If the lower marker is detected, the assay performance is not affected by lower marker or
system peak variability.
Most probable causes Solution
Buffer components of the sample , e.g.
salts, detergents, other additives etc.
may interfere with the lower marker.
Ionic strength of the sample buffer may affect the lower marker intensity. See Kit
Guide for a list of compatible buffers and buffer compounds. For an updated list,
refer to the web site www.agilent.com/genomics/bioanalyzer.
If necessary, dilute, dialyze or desalt the sample. It is recommended to perform a
serial dilution with water or compatible buffer to determine the optimal dilution.
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