Maintenance and Troubleshooting 53
Troubleshooting the DNA Application
5
Symptoms (DNA)
Wavy Baseline
Back to “Symptoms (DNA)” on page 37
Most probable causes Solution
Leak currents due to contaminated
electrodes.
Clean electrodes as described in “How to Clean the Pin Set of the Electrode
Cartridge” on page 126.
Leak currents due to liquid spillage on top of
the chip (detergents in sample buffer lower
surface tension in the wells of the chip).
Prepare a new chip. Lower vortexing speed or mix samples manually.
Dye concentration too low. Use dye concentration according to the Kit Guide. Let the dye warm up to
room temperature for 30 minutes before preparing the gel-dye mix.
Probable causes Solution
Chip not properly primed. Clogged chip
priming station or wrong priming station
settings.
Prepare a new chip. Check the priming station as described in “Checking the
Chip Priming Station for Proper Performance - Seal Test” on page 139.
Clean/replace syringe, gasket and plastic adapter, if necessary. Check if clip
and base plate of priming station are in the correct position (see Kit Guide).
Leak currents due to wet cartridge.
Use
only 350 µL of water in the cleaning chip.
Ensure the humidity in the room is below 70% at 15 – 27 °C (59 – 81 °F).
Least probable causes Solution
Changes in ambient temperature of more
than 5°C during the run.
Place the 2100 Bioanalyzer instrument in a thermally stable environment.
High voltage power supply defective. Check high voltage power supply using the “Overview” on page 30. If the
power supply is defective, contact Agilent Technologies at
www.agilent.com/genomics/contact.
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