104 Maintenance and Troubleshooting
7
Troubleshooting the Protein Application
Symptoms (Protein)
Spikes
Back to “Symptoms (Protein)” on page 89
Most probable causes Solution
Chip, gel-dye mix, destaining solution,
or electrodes contaminated.
Prepare new chip with new gel-dye mix and new destaining solution.
Wear powder-free gloves only.
Do not touch the underside of the chip.
Do not touch the wells of the chip.
Clean the electrodes as described in “Maintenance of the Electrode
Cartridge” on page 115.
Load the chip immediately after taking it out of its sealed bag.
Gel-dye mix or destaining solution not
properly prepared.
Refer to the Kit Guide for proper preparation of the gel-dye mix and destaining
solution. Let the dye warm up to room temperature for 30 minutes before
preparing the gel-dye mix. Protect the dye from light during this time.
Probable Causes Solution
Vibration of 2100 Bioanalyzer
instrument.
Do not touch the 2100 Bioanalyzer instrument during a run.
Remove vibration devices, such as vortexers and vacuum pumps, from the
bench.
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