Maintenance and Troubleshooting 85
Troubleshooting the RNA Application
6
Symptoms (RNA)
Late Migration
Back to “Symptoms (RNA)” on page 69
Most probable causes Solution
Chip not properly primed. Clogged chip
priming station or wrong priming
station settings.
Check chip priming station as described in “Checking the Chip Priming Station
for Proper Performance - Seal Test” on page 139. Clean/replace syringe, gasket
and plastic adapter, if necessary. Check if clip and base plate of priming station
are in the correct position (see Kit Guide).
Vortex speed too high. Vortex chip for 1 minute.
Only use the IKA vortexer. Ensure speed is adjusted to the set point.
Leak currents due to contaminated
electrodes.
Clean electrodes as described in “How to Clean the Pin Set of the Electrode
Cartridge” on page 126.
Leak currents due to liquid spillage on
top of the chip (detergents in sample
buffer lower surface tension in the
wells of the chip).
Prepare a new chip. Lower vortexing speed or mix samples manually.
Probable causes Solution
Vortex adapter not connected tightly. Press vortex adapter tightly on mount (vortex adapter must not rock).
Replace vortex adapter as described in “Changing the Adapter” on page 150.
Loss of gel separation properties. Gel or gel-dye mix expired or stored incorrectly. Check Kit Guide for proper
storage of gel and gel-dye mix.
Use gel-dye mix within indicated time. Do not use expired reagents.
To Next Page
Loading...
Need help?
General
