Maintenance and Troubleshooting 121
Maintenance of the Electrode Cartridge
8
RNA Nano Assay
Cleaning the Electrodes after each RNA Nano Assay
When the assay is complete, immediately remove the used chip from the 2100
Bioanalyzer instrument and dispose of it according to good laboratory
practice. Then perform the following procedure to ensure that the electrodes
are clean and no residues are left over from the previous assay.
1 Slowly fill one of the wells of the electrode cleaner with 350 μL RNase free
water.
2 Open the lid and place electrode cleaner in the 2100 Bioanalyzer
instrument.
3 Close the lid and leave it closed for about 10 seconds.
4 Open the lid and remove the electrode cleaner.
5 Wait another 10 seconds for the water on the electrodes to evaporate.
When After each RNA Nano run.
Parts required # p/n Description
1 NA Electrode cleaner (required amount included in the kits)
1 NA RNase-free water
Use a new electrode cleaner with each new kit.
Leak currents between electrodes
Liquid spill may cause leak currents between the electrodes.
➔ Never fill more than 350 µL of water in the electrode cleaner.
Remove the RNase-free water from the electrode cleaner at the end of the day.