72 Maintenance and Troubleshooting
6
Troubleshooting the RNA Application
Symptoms (RNA)
Chip Not Detected
Back to “Symptoms (RNA)” on page 69
Most probable causes Solution
Insufficient volume in well(s) or chip is
empty.
Check Kit Guide for the amount of liquid to be pipetted. Ensure all wells contain
ladder, samples or buffer.
Chip not properly primed. Air bubble in
chip.
Prepare a new chip. Check chip priming station as described in “Checking the
Chip Priming Station for Proper Performance - Seal Test” on page 139.
Clean/replace syringe, gasket and plastic adapter, if necessary.
Check if clip and base plate of priming station are in the correct position (see Kit
Guide).
Probable causes Solution
No communication between the
instrument and PC.
Test the PC-instrument communication as described in “Verify the Instrument
Communication” on page 16.
Least probable causes Solution
High voltage power supply defective. Check high voltage power supply using the “Overview” on page 30. If the power
supply is defective, contact Agilent Technologies at
www.agilent.com/genomics/contact.
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