Maintenance and Troubleshooting 73
Troubleshooting the RNA Application
6
Symptoms (RNA)
Run Aborted
In the logbook, an error will appear:
Run aborted on port x. Instrument error occurred on port
x, Unusual high or low voltage or current was detected during the start phase of the on-chip
analysis.
The marked wells will indicate the wells on the chip that caused the problem.
Most probable causes Solution
Insufficient volume in well(s). Check Kit Guide for the amount of liquid to be pipetted. Ensure all wells contain
sufficient ladder, samples or buffer.
Air bubble at the bottom of the well,
obstructing access to microchannels.
Always insert the pipette tip to the bottom of the well when dispensing the
liquid. Remove large air bubbles with a pipette tip (small bubbles on top of the
well will not affect the assay).
Dirty electrodes. Clean electrodes according to instructions in “How to Clean the Pin Set of the
Electrode Cartridge” on page 126.
Chip not properly primed. Clogged chip
priming station or wrong priming
station settings.
Prepare a new chip. Check chip priming station as described in “Checking the
Chip Priming Station for Proper Performance - Seal Test” on page 139.
Clean/replace syringe, gasket, and plastic adapter, if necessary.
Check if clip and base plate of priming station are in the correct position (see Kit
Guide).
Probable causes Solution
Chip prepared with cold reagents. Prepare a new chip. Allow all reagents and reagent mixes to warm up to room
temperature for 30 minutes prior to use.
Store chips at room temperature.
Least probable causes Solution
High voltage power supply defective. Check high voltage power supply using the “Overview” on page 30. If the power
supply is defective, contact Agilent Technologies at
www.agilent.com/genomics/contact.
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