Maintenance and Troubleshooting 39
Troubleshooting the DNA Application
5
Symptoms (DNA)
Too Low Quantitation Results
Back to “Symptoms (DNA)” on page 37
Most probable causes Solution
Pipetting error during preparation of
reagent mixes.
Check dilution procedure and calibration of pipette.
Chip pipetting error. Prepare new chip. Always insert the pipette tip to the bottom of the well when
dispensing the liquid. Use appropriate pipette and tips.
Insufficient vortexing of chip. Vortex chip for 1 minute. Only use the IKA vortexer. Adjust the speed to the
set-point.
Probable causes Solution
Loaded chip kept for too long before
run.
Prepared chips must be used within 5 minutes.
Dye concentration too high. Use dye concentration according to the Kit Guide. Let the dye warm up to room
temperature for 30 minutes before preparing the gel-dye mix.
Least probable causes Solution
Sample concentration too high. Use sample concentration according to the Kit Guide.
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