Maintenance and Troubleshooting 91
Troubleshooting the Protein Application
7
Symptoms (Protein)
Too Low Quantitation Results
Back to “Symptoms (Protein)” on page 89
Most probable causes Solution
Alignment of upper marker not set
properly.
Manually set upper marker. Follow instructions for “Manual Marker
Assignment” on page 96.
Pipetting error during preparation of
reagent mixes or chip.
Refer to the Kit Guide for proper preparation of reagents.
Check dilution procedure and calibration of pipette.
Chip preparation error. Prepare new chip. Always insert the pipette tip to the bottom of the well when
dispensing the liquid. Use appropriate pipette and tips.
Probable causes Solution
Sample concentration too high. Use sample concentration according to the specifications in the Kit Guides. Do
not forget to dilute samples with deionized water after heat denaturation.
Diluted samples are degraded. Use diluted samples within one day. Store samples at 4°C when not in use for
longer than 1 hour.
Least probable causes Solution
Loaded chip kept too long before run. Prepared chips must be used within 5 minutes of preparation.
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