114 Maintenance and Troubleshooting
7
Troubleshooting the Protein Application
Symptoms (High Sensitivity Protein)
Low Signal Intensity
Back to “Symptoms (High Sensitivity Protein)” on page 112
Most probable causes Solution
Insufficient labeling of ladder or
samples.
Follow instructions in the Kit Guide.
Labeling occurs between pH 8-9.
Proteins must contain lysines for labeling reaction.
Check buffer compatibility in the “Compatibility List for the Labeling Reaction” in
the Kit Guide.
Insufficient sample present. Follow instructions for protein concentration in the Kit Guide.
Low abundant proteins may require pico labeling (Technical Note 5990-3703EN).
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