Invitrogen EVOS M5000 - Page 46

3.
To identify targets, click Target, then click and drag to draw a circle (blue) around a representative
target.
4.
If needed, click Target again to identify other targets (for example, nuclei that might appear
dierent) to improve the accuracy of your count.
Note: For best results, follow these guidelines when identifying target objects:
·
When selecting objects, circle the entire object and include a slight border around it.
·
To include objects of lower intensity in your count, select dimmer objects during identification.
·
Circle only one object at a time to help define object size for segmentation.
Chapter5Measure, annotate, and analyze captured images
Count cells – auto count
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EVOS
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M5000 Imaging System User Guide