Invitrogen EVOS M5000 - Page 49

7.
To count closely grouped cells that are touching or overlapping as distinct objects, select from the
Split Cells options:
•
None:
Touching or overlapping objects are not counted separately.
•
Shape: Distinct objects are identified and counted based on shape.
•
Intensity: Distinct objects are identified and counted based on pixel intensity.
8.
The
Refine section displays a histogram plot showing Count versus Intensity, Area, or Circularity.
In this example, Intensity is selected.
9.
To
refine your count, select Intensity, Area, or Circularity, move the gate handles to set the upper
or the lower boundary for the selected parameter.
You can refine the count by a single parameter or by multiple parameters.
The software applies the selected boundaries and recalculates the count.
Chapter5Measure, annotate, and analyze captured images
Count cells – auto count
5
EVOS
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M5000 Imaging System User Guide
49