Invitrogen EVOS M5000 - Page 67

10.
Define the Z-Stack parameters to determine the number of “optical sections” captured in the
Z-Stack image set:
•
Automatically compute Z-Stack parameters: Select to calculate the number of images and
their Z-positions automatically based on the Z-Stack boundaries and the default Step Size.
•
Manually Set Step Size: Select to enter the Step Size (Z-distance in µm between focal
planes) in the corresponding text box. The system calculates the number and Z-positions of
the images based on your entry.
•
Manually Set Number of Images: Select to enter the Number of Images (Z-distance in µm
between focal planes) in the text box. The system calculates the Step Size and the Z-positions
of the images based on the number of images.
11.
Click Generate Projection Image
to generate a projection image.
When selected, the system uses the most in-focus pixels from images captured at dierent focal
planes to generate a composite in-focus image. Each image in the Z-Stack is also separately
saved.
When unselected, the system only saves the individual Z-Stack images.
12.
Click
Next to set up a Run.
13.
Enter the Total Time
for Run 1in Hours, Minutes, and Seconds.
You can create multiple Runs for a Time Lapse Routine, each with its own duration and image
capture frequency.
Chapter7Capture time lapse images
Run a time lapse routine
7
EVOS
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M5000 Imaging System User Guide
67