Invitrogen EVOS M5000 - Page 68

14.
Set the Image capture options for Run 1. Available options are Frequency, Intervals, and As fast
as possible.
•
Frequency: Enter the time in Hours, Minutes, and Seconds that must elapse before a new
set of images are captured.
For example, in an experiment with an image capture frequency of 2 minutes and 30seconds,
the images will be captured every 2 minutes and 30 seconds after the initial set of images are
acquired at time point 0.
•
Intervals: Enter the total number of time intervals between the captured image sets for a
given run duration.
Images are collected at the end of an interval. For example, in a Run experiment with a
duration of 5 minutes and 2 intervals, the images will be captured every 2 minutes and
30 seconds after the initial set of images are acquired at time point 0.
•
As fast as possible: Select this option to capture a new set of images immediately after
completing the previous set without any delay between the sets.
The speed with which the images are captured depends on the specific choices made for the
routine such as the autofocus frequency and exposure settings.
IMPORTANT! To reduce phototoxicity, minimize the capture frequency and light intensity, and
maximize gain.
15.
If you have opted to use the EVOS
™
Onstage Incubator in your time lapse routine, set the
Incubator options for Run 1.
a.
Enter the target values for Temperature, CO
2
, and Oxygen.
b.
If desired, select
Humidity to use a humidified atmosphere in the incubator.
c.
Select the
Shutdown option for the incubator.
•
Turn o manually: The incubator remains on until the Use Incubator option is manually
deselected.
•
Turn o at the end of experiment: Heat, humidity, and the flow of gas are automatically
turned o at the end of the experiment.
•
Turn o after: Enter the time period in Hours and Minutes that must elapse before the
heat, humidity, and the flow of gas are automatically turned o.
16.
(Optional) Click Add Run to add another run to the routine, then define the Run options as
described for Run 1.
17.
When
finished with adding Runs, click Next to set the Save options.
18.
Click (
Choose Save Folder), navigate to the desired destination folder for the Time Lapse
images, then click Select Folder.
19.
Select the
File Type: PNG or TIFF.
20.
If desired, select
Save Time Lapse Movie to create a video from the images captured in the time
lapse routine.
21.
Select the
Video Type: AVI or MP4.
Chapter7Capture time lapse images
Run a time lapse routine
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EVOS
™
M5000 Imaging System User Guide