80 Maintenance and Troubleshooting
6
Troubleshooting the RNA Application
Symptoms (RNA)
Broad Peaks
Back to “Symptoms (RNA)” on page 69.
Most probable causes Solution
Sample contaminated with genomic
DNA.
Check RNA isolation protocol. To remove genomic DNA, perform DNase
treatment.
Leak currents due to contaminated pin
set.
Clean the pin set of the electrode cartridge. Follow cleaning procedure, see “How
to Clean the Pin Set of the Electrode Cartridge” on page 126
Probable causes Solution
Chip not properly primed. Clogged chip
priming station or wrong priming
station settings.
Prepare a new chip. Check the priming station as described in “Checking the
Chip Priming Station for Proper Performance - Seal Test” on page 139.
Clean/replace syringe, gasket and plastic adapter, if necessary.
Check if clip and base plate of priming station are in the correct position (see Kit
Guide).
Least probable causes Solution
Dye concentration too high. Use dye concentration according to the Kit Guide. Let the dye warm up to room
temperature for 30 minutes before preparing the gel-dye mix. Protect the dye
from light during this time.
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