Chapter 4: Instrument QC 63
For lyse/wash (LW) or lyse/no-wash (LNW) assays, the software
automatically performs 2-, 3-, or 4-color setup based on the BD Calibrite
bead lot IDs and the bead populations detected.
; Tip For consistency, run daily QC using the same assay and the maximum
number of colors needed. Instrument settings for 4-color LNW are also valid
for 3- and 2-color LNW assays, as long as all use the same fluorochromes
for FL1, FL2, FL3, and FL4.
NOTICE You must have a valid LW Calib File (not Calib File. PerCP-Cy5.5)
to perform HLA-B27 calibration.
4 Under CaliBRITE Bead Lot IDs, enter the IDs supplied with the current lot
of beads.
Enter each ID exactly as shown (five numbers and one capital letter). Do
not add a space between the numbers and letter.
Enter the correct lot IDs for the assays to be run. The APC Lot ID is
required only for 4-color setup. If APC beads are detected and you do not
enter an APC lot ID, 3-color setup will be performed.
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