64 BD FACSCalibur Instructions for Use
5 Under HLA-B27 Calibration Lot IDs (if appropriate), enter the bead and
reagent lot IDs and suffixes.
NOTICE Rerun HLA-B27 calibration each time you enter new bead lot IDs
and suffixes. Information at the Set Up view must correspond to the
current HLA-B27 calibration file.
6 Under Automatic Saving Options, click the checkbox for each Summary
Report you want to save; click the Location button to change the default
name or storage location.
A dialog appears where you can enter a new name or different storage
location. For file types and default names and locations, see page 34.
7 Under Lyse/Wash or Lyse/No-Wash settings, if you know your application’s
usual threshold value or gain, enter them in the corresponding fields.
The values are not used by BD FACSComp software, but are saved in the
corresponding calibration file. When the settings are downloaded to the
cytometer, these values can help facilitate setup.
8 Prepare BD Calibrite beads as directed in the reagent insert.
NOTICE For calibrations using PerCP-Cy5.5 beads, dilute beads in Bead
Dilution Buffer.
For LW and LNW assays, BD FACSComp software uses unlabeled beads
for automatic PMT adjustment (unlabeled + APC beads for four-color
setup), and mixed beads for automatic compensation adjustment and the
Sensitivity Test.
For accurate results, make sure target values are appropriate for the current
lot of beads. BD Biosciences will notify you when target values need editing
because of a change in bead raw material. See page 65.
Make sure the correct information is entered per run. This information is
critical for providing accurate results. Only one lot ID can be entered per
Schedule document.
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