Chapter 4: Instrument QC 75
After manual adjustment:
• Click Initial Comp and then Start to revert to the automatic settings, or
• Click Next to save settings and proceed.
Note the following about compensation settings:
• Fluorescence mean channel differences can fluctuate slightly after
compensation values are set.
• Bead populations might not be perfectly aligned vertically and horizontally.
For example, FL3–%FL2 might appear undercompensated even though its
bead positioning is appropriate for placing cell populations in the correct
locations.
• Because of different time-delay calibration values, FL3–%FL4 and FL4–
%FL3 compensation values can vary considerably from one run to the
next. For example, FL4–%FL3 might be 14% for one setup and 7% for the
next. Both setups provide compensation values appropriate for your
application.
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