Invitrogen EVOS M5000 - Page 60

3.
To view the pure fluorescence signal and to observe the various levels of fluorescence marker
(GFP) expression, uncheck the Transmitted Light Channel.
4.
Select
Threshold Mask to highlight the fluorescence areas included in the Transfection Eciency
calculation.
Chapter5Measure, annotate, and analyze captured images
Measure confluence
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60
EVOS
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M5000 Imaging System User Guide