7. Hardware Validation
7-22
CBM-20A/20Alite
6
Inject 10µL of mobile phase.
Verify that no peaks are observed.
7
Create a sequence table based on the conditions shown in the following table.
For details on creating sequence tables, refer to "4.3 Creating Sequence Files" P. 4-22.
When using a manual injector, inject a volume equal to 5 times the loop volume.
8
Use the following formula to obtain the retention time, peak area, and peak height for
each of 6 consecutive analyses.
Parameters Set value
Injection Volume 10µL
Injections/Vial 6 times
Run Time 5 min
• CHECK
CRITERIA
• The RSD (C.V.)'s obtained must satisfy the following
criteria:
• Retention time RSD must not exceed 0.5 %.
• Peak area RSD must not exceed 1.0 %.
RSD(C.V.)=(SD/X) x 100
SD =
X = (X1 + X2 •••• Xn - 1 + Xn)/n
n : Number of analyses
X1••Xn : Retention time (or areas) of each peak
X : Average
SD : Standard deviation
RSD : Relative standard deviation
C.V. : Coefficient of variation
(Xi-X)
2
n-1
n
i =1
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