5. Application Operation
5-22
CBM-20A/20Alite
2
Set the method parameters for the fraction collector.
For details on the setting ranges, minimum units, units, and default values for the
parameters, refer to "5.12.5 Fraction Collector" P. 5-109. For details on the functions of the
buttons, refer to "4.2.2 Editing the "Method Parameter" Window" P. 4-12.
For parameters such as the vial volume, set values that match the operating
environment. For example: when set to Vial volume: 0.01 mL, Flow rate: 10 mL/
min, the fraction time will be 60 mS per vial (0.01 [ml]/10 [ml/min] = 0.001 [min]),
which will be too short to actually allow fractioning. Set the parameters to allow for
intervals of at least 2 to 3 seconds or more.
3
Click when setting is complete.
The "Method Parameter" window closes.
5.6.3 Controlling the Fraction Collector with a Time Program
The fraction collector can be controlled with a time program in the same way as other
components.
For details on how to set time programs, refer to "4.4 Gradient Analysis Using Time Programs" P.
4-28. For details on the commands, setting ranges, and default values for the fraction collector,
refer to "5.13 List of Time-program Commands" P. 5-115.
When setting fraction-collector commands in a time program, be sure that the time set
for the first fraction-collector command is at least 0.33 minutes.
5.6.4 Setting the Number and Positions of Vials in Racks
If using a fraction collector rack other than a Shimadzu rack, the number and positions of vials
must be stored in the system. Enter the rack information in the "Fraction Collector Rack
Adjustment" window.
Displaying the "Fraction Collector Rack Adjustment" Window
1
Click in the "Analysis" tab page.
The "Fraction Collector" tab page in the "Method Parameter" window is displayed.
2
Select the desired rack ("User Rack" 6 to 9) from the [Collection Rack No.] pull-down
menu.
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