4.4 Gradient Analysis Using Time Programs
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CBM-20A/20Alite
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4.4.4 Time-program Setting Example
A setting example for time programs is provided below.
Gradient mode: Binary gradient (B.GE1)
Change in concentration: After 10 minutes have elapsed, the concentration of mobile phase B
is changed to 80 % and is held at 80 % until a total of 15 minutes
have elapsed. After a total of 15.01 minutes have elapsed, the
concentration of mobile phase B is reduced to 0 %.
One cycle is 20-minutes long.
The setting for mobile phase B (B.CONC) in the method is 0 %.
Fig. 4.1
Set the time program for this operation in the way shown in the following table.
Be sure to set the STOP command for the "Controller" at the end of the time program.
No. Time Module Function Value
1 10 B. GE1 B. CONC 80.0
2 15 B. GE1 B. CONC 80.0
3 15.01 B. GE1 B. CONC 0.0
4 20 Controller STOP