Maintenance and Troubleshooting 83
Troubleshooting the RNA Application
6
Symptoms (RNA)
Wavy Baseline
Most probable causes Solution
Contamination with genomic DNA. Check RNA isolation protocol. To remove genomic DNA, perform DNase
treatment.
Leak currents due to contaminated
electrodes.
Clean the electrode cartridge as described in “How to Clean the Pin Set of the
Electrode Cartridge” on page 126.
Prepare a new chip.
Leak currents due liquid spillage on
top of the chip (detergents in sample
buffer lower surface tension in the
wells of the chip).
Prepare a new chip. Lower vortexing speed or mix samples manually
Chip not properly primed. Clogged
priming station or wrong priming
station settings.
Check chip priming station as described in “Checking the Chip Priming Station
for Proper Performance - Seal Test” on page 139. Clean/replace syringe, gasket
and plastic adapter, if necessary.
Check if clip and base plate of priming station are in the correct position (see Kit
Guide).
Probable causes Solution
Leak currents due to wet cartridge.
Use only 350 μL of water in the cleaning chip.
Ensure the
humidity in the room is below 70% at 15-27°C (59-81°F).
To Next Page
Loading...
Need help?
General
