Applied Biosystems QuantStudio 6 Flex - Preparing an Array Card for Instrument Verification

b.
Centrifuge the reaction plate for 2 minutes at < 1500 rpm.
IMPORTANT! The reaction plate must be well mixed and centrifuged.
c.
Conrm that the liquid is at the boom of each w
ell of the reaction plate. If
not, centrifuge the reaction plate again at a greater rpm and for a longer
period of time.
IMPORTANT! Do not allow the boom of the plate to become dirty. Fluids
and other contaminants that adhere to the boom of the reaction plate can
contaminate the sample block and cause an abnormally high background.
IMPORTANT! Perform the following procedure only if you are verifying the
performance of a QuantStudio
™
7 Flex System with an array card sample block.
Required materials
•
TaqMan
®
Array Card Sealer
• Centrifuge with centrifuge array card carrier clips and buckets
• Powder-free gloves
• Safety goggles
• Pipeor, 200-µL (with pipee tips)
• RNase P Array Card Instrument Verication Reagents Kit:
– TaqMan
®
Array Card
– RNase P Array Card Instrument Verication Reagents Kit, including tubes
with reagent mix for each port (8 tubes total)
Preparing the RNase P array card
IMPORTANT! Wear powder-free gloves while preparing the array card.
1.
Remove the Array Card RNase P Kit from the freezer, then allow it to warm to
room temperature (for approximately 15 minutes).
IMPORTANT! Y
ou must ll and run the RNase P array card within 60 minutes of
thawing the Array Card RNase P Kit.
2.
Remov
e an array card from its box and place it on a clean, dry surface.
Preparing an
arr
ay card for
instrument
verification
Chapter 2 Calibrate the instrument
Verify the instrument performance
2
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
65