b.
Centrifuge the reaction plate for 2 minutes at < 1500 rpm.
IMPORTANT! The reaction plate must be well mixed and centrifuged.
c.
Conrm that the liquid is at the boom of each w
ell of the reaction plate. If
not, centrifuge the reaction plate again at a greater rpm and for a longer
period of time.
IMPORTANT! Do not allow the boom of the plate to become dirty. Fluids
and other contaminants that adhere to the boom of the reaction plate can
contaminate the sample block and cause an abnormally high background.
IMPORTANT! Perform the following procedure only if you are verifying the
performance of a QuantStudio
™
7 Flex System with an array card sample block.
Required materials
•
TaqMan
®
Array Card Sealer
• Centrifuge with centrifuge array card carrier clips and buckets
• Powder-free gloves
• Safety goggles
• Pipeor, 200-µL (with pipee tips)
• RNase P Array Card Instrument Verication Reagents Kit:
– TaqMan
®
Array Card
– RNase P Array Card Instrument Verication Reagents Kit, including tubes
with reagent mix for each port (8 tubes total)
Preparing the RNase P array card
IMPORTANT! Wear powder-free gloves while preparing the array card.
1.
Remove the Array Card RNase P Kit from the freezer, then allow it to warm to
room temperature (for approximately 15 minutes).
IMPORTANT! Y
ou must ll and run the RNase P array card within 60 minutes of
thawing the Array Card RNase P Kit.
2.
Remov
e an array card from its box and place it on a clean, dry surface.
Preparing an
arr
ay card for
instrument
verification
Chapter 2 Calibrate the instrument
Verify the instrument performance
2
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
65
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