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Applied Biosystems QuantStudio 6 Flex User Manual

Applied Biosystems QuantStudio 6 Flex
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The QuantStudio
6 or 7 Flex System passes the installation specication if the
inequality holds and the instrument successfully distinguishes betw
een unknown
populations A and B with a statistical condence level of 99.7%.
As shown in the following table, you can omit a limited number of outlier wells from
the unknown populations to meet the installation specication.
Sample block
Maximum number of outlier wells that can be removed
Unknown
population A
[1]
Unknown
population B
[2]
Standards
(STD)
[3]
No template
controls (NTC)
96-well plate
[4]
6 6 1 0
384-well plate 10 10 2 0
Array card
[5]
4 4 4 0
[1]
5
,000-copy population for 384-well plates; 800-copy population for array cards.
[2]
10,000-copy population for 384-well plates; 1,600-copy population for array cards.
[3]
Maximum number of wells that can be removed from
each
standard population.
[4]
Standard 96-well plates or Fast 96-well plates
[5]
QuantStudio
7 Flex System only.
Materials required
RNase P Instrument Verication Plate
Centrifuge with plate adapter
Powder-free gloves
Safety goggles
Prepare the plate
IMPORTANT! When performing the RNase P instrument verication test:
·
Perform all calibrations beforehand.
·
Run the RNase P plate soon after you allow the plate to thaw. Minimizing the time
that the prepared plate or array card sits on the bench ensures optimal
performance.
·
Wear powder-free gloves and safety glasses when you prepare plates.
1.
Obtain the RNase P Instrument V
erication Plate from the freezer, then allow the
plate to warm to room temperature (for approximately 5 minutes).
IMPORTANT! Use the RNase P plate within 30 minutes of defrosting it. Until
you are ready to run the plate, store it in the dark and at ambient temperature
(15–30). Do not remove the plate from its packaging until you are ready to run
it.
2.
Remov
e the RNase P plate from its packaging.
3.
Briey vortex and centrifuge the RNase P plate:
a.
Vortex the plate for 5 seconds.
Installation
specification
Prepare the
RNase P plat
e
Chapter 2 Calibrate the instrument
Verify the instrument performance
2
64
QuantStudio
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide

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Applied Biosystems QuantStudio 6 Flex Specifications

General IconGeneral
BrandApplied Biosystems
ModelQuantStudio 6 Flex
CategoryLaboratory Equipment
LanguageEnglish

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