Applied Biosystems QuantStudio 6 Flex - Perform the Uniformity Calibration

c.
Conrm that the liquid in each well of the ROI calibration plate is at the
boom of the well. If not, centrifuge the plate again at a higher rpm and for
a longer period of time.
1.
In the QuantStudio
™
Real-Time PCR Software Home tab, click Instrument
Console.
2.
In the Instrument Console, select the icon for your instrument, then click
Manage Instrument.
Note: If the Manage Instrument buon is inactive, add your instrument to the
My Instruments group.
3.
From the Instrument Manager, start the calibration:
a.
Click
Maintenance, then click Uniformity.
b.
From the Uniformity Calibration screen, click Start Calibration.
4.
Click Next, then prepare for the calibration as instructed.
5.
At the boom of the Setup tab, enter the reagent information for the ROI plate or
array card that y
ou are using.
6.
Load the calibration plate or array card into the instrument:
a.
From the instrument touchscreen, tap to eject the instrument tray.
Perform the
uniformity
calibration
Chapter 2 Calibrate the instrument
Uniformity calibration
2
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
51