Applied Biosystems QuantStudio 6 Flex - Perform the Background Calibration

2.
Remove the background calibration plate from its packaging. Do not remove the
optical lm.
3.
Vortex and centrifuge the plate:
a.
Vortex the background calibration plate for 5 seconds.
b.
Centrifuge the plate for 2 minutes at <1500 rpm.
IMPORTANT! The background calibration plate must be w
ell mixed and
centrifuged.
c.
Conrm that the liquid in each well of the background calibration plate is at
the boom of the well. If not, centrifuge the plate again at a higher rpm and
for a longer period of time.
IMPORTANT! Do not allow the boom of the plate to become dirty. Fluids
and other contaminants that adhere to the plate boom can contaminate the
sample block and cause an abnormally high background signal.
1.
In the QuantStudio
™
Real-Time PCR Softw
are Home tab, click Instrument
Console.
2.
In the Instrument Console, select the icon for your instrument, then click
Manage Instrument.
Note: If the Manage Instrument buon is inactive, add your instrument to the
My Instruments group.
3.
In the Instrument Manager, start the calibration:
a.
Click
Maintenance, then click Background.
b.
In the Background Calibration screen, click Start Calibration.
4.
Click Next, then prepare for the calibration as instructed.
Perform the
back
ground
calibration
Chapter 2 Calibr
ate the instrument
Background calibration
2
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
47