Applied Biosystems QuantStudio 6 Flex - APPENDIX F Calibration consumable preparation; Create a background plate or array card

Calibration consumable preparation
■
Create a background plate or array card ................................ 151
■
Create a custom dye plate for calibration ............................... 153
Create a background plate or array card
Whenever possible, use a Background Plate or the TaqMan
®
Array Background Buer
that is included with the spectral calibration kit. The plates/array cards supplied in the
kit contain a buer that accurately simulates the reagents used for PCR, and,
therefore, produces high-quality calibration data. If a background plate or array card
from a spectral calibration kit is not available, you can create one as described below.
96/384-Well plate sample block
• MicroAmp
™
Optical 96/384-Well Reaction Plate
• Safety glasses
• Optical Adhesive Cover or Optical Flat Caps
• Pipeor, 200-µL (with pipee tips)
• Powder-free gloves
• Deionized water
TaqMan
®
Array Card block (QuantStudio
™
7 Flex System only)
• TaqMan
®
Array Cards
• TaqMan
®
Array Card Sealer
• Centrifuge with array card buckets and array card carrier clips
• Permanent marker or pen
• Pipeor, 200-µL (with pipee tips)
• Powder-free gloves
• Safety glasses
• Deionized water
F
Required
materials
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
151