Applied Biosystems QuantStudio 6 Flex User Manual

Note: Before starting the calibration, the instrument may pause (up to
10 minutes) to allow the heated cov
er to reach the required temperature.
8.
When the run is complete and the software displays the Analysis screen, select
each lter from the Filter Set drop-down list, then conrm that the
corresponding ROI Image displays a green circle around each well area.
9.
After you inspect all ROI images, conrm the status of the calibration, where
passed indicates that the run produced viable calibration data, and failed indicates
that the run did not produce data or that the data it collected is unusable.
Analysis status Action
Passed Click Ne
xt.
Failed Troubleshoot the failed calibration (see “Troubleshooting“).
10.
Unload the calibration plate or array card:
a.
After the calibration, tap on the touchscreen to eject the plate or array
card.
Not
e: If the instrument does not eject the plate or array card, remove it as
explained in “Troubleshooting“.
b.
Remove the ROI calibration plate or array card from the instrument tray and
store it in the dark at room temperature until you are ready to perform the
uniformity calibration.
WARNING! PHYSICAL INJUR
Y HAZARD. During instrument
operation, the plate or array card temperature can reach 100°C. Allow
the consumable to cool to room temperature before removing.
c.
T
ap
on the instrument touchscreen to close the instrument tray.
11.
In the ROI Calibration screen, click Finish to complete the calibration, then click
Y
es when prompted to save the results.
Note: If the QuantStudio
™
Real-Time PCR Software displays the warning
Saving ROI calibration will invalidate other existing calibrations except HRM
Calibrations, click OK to proceed.
Note: HRM (High Resolution Melt) is a separately licensed module. If your
instrument requires this module, contact Thermo Fisher Scientic.
12.
(Optional) Click Print Report in the upper right corner of the screen to print a
summary of the calibration results for your records.
Background calibration
The software uses the background calibration data during subsequent experiments to
remove background uorescence from the experiment data.
Chapter 2 Calibr
ate the instrument
Background calibration
2
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
45

Applied Biosystems QuantStudio 6 Flex Specifications

General

Brand	Applied Biosystems
Model	QuantStudio 6 Flex
Category	Laboratory Equipment
Language	English