Applied Biosystems QuantStudio 6 Flex User Manual

During the ROI calibration, the instrument software captures images of the ROI
calibration plate or array card at each instrument lter
. An ROI calibration passes if
the collected image for each lter distinguishes all wells of the ROI plate or array card.
Each well in the image must be distinct and visible at the same luminosity relative to
the other wells in the image.
Note: You can review the ROI calibration image for each lter set by selecting the
desired lter combination from the Filter Set menu of the ROI tab in the Instrument
Manager.
Passing image Failing image
Green circles around all wells indicate that
the wells calibrated successfully. Each
green circle indicates that the region of
interest for the well position is sufficiently
bright.
Red circles around some of the wells
indic
ate that the wells did not calibrate. The
absence of a circle indicates that the region
of interest for the well position is not
sufficiently bright.
IMPORTANT! Wear powder-free gloves and safety glasses when you prepare plates
or array cards.
Prepare the calibration consumable appropriate for your QuantStudio
™
6 or 7 Flex
System:
•
“Prepare the ROI calibration plate“ on page 42
• “Preparing array cards for instrument calibration“ on page 37
Materials required
• Region of Interest (ROI) Plate
• Centrifuge with plate adapter
• Powder-free gloves
• Safety goggles
About the
calibration data
Preparing the
c
alibration plate
or array card
Prepare the ROI
c
alibration plate
Chapter 2 Calibr
ate the instrument
ROI calibration
2
42
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide

Applied Biosystems QuantStudio 6 Flex Specifications

General

Brand	Applied Biosystems
Model	QuantStudio 6 Flex
Category	Laboratory Equipment
Language	English