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Applied Biosystems QuantStudio 6 Flex User Manual

Applied Biosystems QuantStudio 6 Flex
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Calibration overview
The QuantStudio
6 and 7 Flex Systems require regular calibration and maintenance
for proper operation. To ensure proper operation of your instrument, perform weekly,
monthly, and semiannual maintenance as indicated in the following table.
IMPORTANT! P
erform calibrations and run experiments under the environmental
conditions specied in Appendix H, “Instrument specication and layout“. Exposure
to extreme temperatures can have adverse eects on the run results, as well as
shortening the life span of the instrument components.
IMPORTANT! Do not use organic solv
ents to clean the QuantStudio
6 and 7 Flex
Systems.
Frequency User-performed maintenance task
Weekly
Check the computer disk space. If necessary, archive or back up
y
our experiment files and instrument settings.
Power off the computer that controls the instrument, then power
on the computer after 30 seconds.
Clean the surface of the instrument with a lint-free cloth.
Monthly
Perform a background calibration.
[1]
Run disk cleanup and disk defragmentation.
Perform an instrument self test.
Semi-annually (every
6 months)
Perform an ROI calibration.
Perform a background calibration.
[1]
Perform a uniformity calibration.
Perform a dye calibration.
(TaqMan
®
Array Card block only)
Perform a normalization
calibration.
[2]
As needed
Perform an RNase P instrument verification run.
Replace the instrument lamp.
[3]
[1]
Y
ou can perform a background calibration to check for contamination.
[2]
A normalization calibration is not required for the 96-well plate blocks (0.1-mL or 0.2-mL) or the 384-well
plate block.
[3]
After replacing the instrument lamp, perform all calibrations and an RNase P instrument verification run.
Calibration and
maint
enance
schedule
Chapter 2 Calibrate the instrument
Calibration overview
2
QuantStudio
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
27

Table of Contents

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Applied Biosystems QuantStudio 6 Flex Specifications

General IconGeneral
BrandApplied Biosystems
ModelQuantStudio 6 Flex
CategoryLaboratory Equipment
LanguageEnglish

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