Applied Biosystems QuantStudio 6 Flex User Manual

Verify the instrument performance
When necessary, you can perform the RNase P instrument verication test to conrm
the performance of the QuantStudio
™
6 and 7 Flex Systems.
We recommend performing an RNase P instrument verication test:
• After moving the instrument to another location.
• As needed to conrm the function of the instrument.
The RNase P instrument verication test uses instrument verication kits available
from thermosher.com. The kits dier only in the consumable format for which they
are designed: an RNase P Instrument Verication Plate for QuantStudio
™
6 and 7 Flex
Systems with 96/384-well sample blocks and an Array Card RNase P Kit for the
QuantStudio
™
7 Flex System with array card sample blocks.
RNase P instrument verification plates
The RNase P plate is preloaded with the reagents necessary for the detection and
quantitation of genomic copies of the human RNase P gene (a single-copy gene
encoding the RNase moiety of the RNase P enzyme). Each well contains: PCR master
mix, RNase P primers, FAM
™
dye-labeled probe, and a known concentration of
human genomic DNA template.
Figure 5 illustrates the arrangement of the
standard and unknown populations on a
96-well and Fast 96-well RNase P plate.
The RNase P plate contains ve replicate
groups of standards (1250, 2500, 5000,
10000, and 20000 copies), two unknown
populations (5,000 and 10,000 copies),
and a no template control (NTC).
Figure 6 illustrates the arrangement of the
standard and unknown populations on a
384-well RNase P plate. The RNase P
plate contains ve replicate groups of
standards (1250, 2500, 5000, 10000, and
20000 copies), two unknown populations
(5,000 and 10,000 copies), and a no
template control (NTC).
When to perform
the test
About the RNase P
kits
Figure 5 96-w
ell and Fast 96-well RNase P
plate layout
Figure 6 384-w
ell RNase P plate layout
Chapter 2 Calibr
ate the instrument
Verify the instrument performance
2
62
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide

Applied Biosystems QuantStudio 6 Flex Specifications

General

Brand	Applied Biosystems
Model	QuantStudio 6 Flex
Category	Laboratory Equipment
Language	English