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Applied Biosystems QuantStudio 6 Flex User Manual

Applied Biosystems QuantStudio 6 Flex
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Array card RNase P kits
The RNase P Kits include one empty array card and eight tubes of solution. Each tube
contains reaction mix (PCR master mix, RNase P primers, and FAM
-MGB dye-
labeled probe) and a known concentration of human genomic DNA template.
To perform an instrument verication
run, each solution is loaded into the
empty array card in the arrangement
shown in Figure 7. When complete, the
array card contains ve replicate groups
of standards (200, 400, 800, 1600, and
3200 copies), two of unknown
populations (800 and 1600 copies), and
one that serves as a no template control
(NTC).
The software performs the same analysis of data from an instrument verication run
regardless of the type of consumable used.
After the run, the software:
1.
Generates a standard curve from the averaged threshold cycle (C
t
) values of the
replicate groups of standards.
2.
Calculates the concentration of the two unknown populations using the standard
curve.
3.
Calculates the following to assess the QuantStudio
6 or 7 Flex System
performance:
[(CopyUnk
2
) – 3(σ
CopyUnk2
)] > [(CopyUnk
1
) + 3(σ
CopyUnk1
)]
where:
CopyUnk
1
= Average copy number of unknown population A
σ
CopyUnk1
= Standard deviation of unknown population A
CopyUnk
2
= Average copy number of unknown population B
σ
CopyUnk2
= Standard deviation of unknown population B
Note: Unknown population A refers to the 5,000-copy population and unknown
population B refers to the 10,000-copy population in the RNase P plate.
Figure 7 RNase P s
tandards array card
layout
Analytical
performance
Chapter 2 Calibr
ate the instrument
Verify the instrument performance
2
QuantStudio
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
63

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Applied Biosystems QuantStudio 6 Flex Specifications

General IconGeneral
BrandApplied Biosystems
ModelQuantStudio 6 Flex
CategoryLaboratory Equipment
LanguageEnglish

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