Applied Biosystems QuantStudio 6 Flex - Run the Rnase P Plate or Array Card

d.
Dispense the uid so that it sweeps in and around the ll reservoir toward
the vent port. Pipet the entire 100 µL into the reservoir, but do not go past the
rst stop of pipeor plunger or you may blow the solution out of the port.
IMPORTANT! Do not allow the tip to contact and possibly damage the
coated foil beneath the ll port.
GR2159
Microfluidic Card
Filling the card
5.
Centrifuge and seal the array card as explained in step 6 through step 8
in “Fill
the calibration TaqMan
®
Array Cards“ on page 37.
6.
Run the prepared array card as soon as possible after lling it.
IMPORTANT! Store the array card in a dark place until y
ou are ready to run it. The
uorescent dyes in the array card are photosensitive. Prolonged exposure to light can
diminish the uorescence of the dye.
1.
In the QuantStudio
™
Real-Time PCR Softw
are Home tab, click Instrument
Console.
2.
In the Instrument Console, select the icon for your instrument, then click
Manage Instrument.
Note: If the Manage Instrument buon is inactive, add your system to the My
Instruments group.
3.
In the Instrument Manager, start the RNase P run:
a.
Click
Maintenance, then click RNase P Run.
b.
In the RNase P Run screen, click Start RNase P Run.
4.
Click Next, then prepare for the instrument v
erication test as instructed.
5.
At the boom of the Setup tab, enter the reagent information for the RNase P
plate or array card that you are using.
Run the RNase P
plat
e or array card
Chapter 2 Calibr
ate the instrument
Verify the instrument performance
2
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
67