Applied Biosystems QuantStudio 6 Flex - Page 38

3.
Mark the side of the empty array
cards with: Background, FAM
™
, ROI,
ROX
™
, VIC
™
, FAM
™
/ROX
™
, and
VIC
™
/ROX
™
.
4.
For each array card, pipet 100 µL of
the appropriate calibration solution
into each of the eight reservoirs in
the array card:
a.
Place the array card on a lab bench, with the foil side down.
b.
Load 100 µL of the calibration solution into a pipee.
c.
Hold the pipee in an angled position (approximately 45 degrees) and place
the tip into the ll port. The ll port is the larger of the two holes on the left
side of the ll reservoir.
GR2158
Microfluidic Card
Card Ports detail
21
1
Fill port
2
Vent port
d.
Dispense the uid so that it
sw
eeps in and around the ll
reservoir toward the vent port.
Pipet the entire 100 µL into the ll
reservoir, but do not go past the
rst stop of pipeor plunger or
you may blow the solution out of
the port.
IMPORTANT! Do not allow the
tip to contact and possibly
damage the coated foil beneath
the ll port.
5.
Repeat step 4 to ll the remaining array card with the appropriate calibration
reagents.
6.
Centrifuge the array card(s):
a.
Place the lled array card(s) into a centrifuge array card carrier clip and
place empty array cards in the remaining slots. Conrm that the labels on
the buckets and clips are oriented in the same direction.
Mark
here
GR2159
Microfluidic Card
Filling the card
Chapter 2 Calibrate the instrument
Preparing array cards for instrument calibration
2
38
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide