Applied Biosystems QuantStudio 6 Flex User Manual

12.
Repeat the calibration to calibrate the instrument for the remaining dyes that are
present in the experiments that you will be running.
13.
(Optional) Click Print Report in the upper right corner of the screen to print a
summary of the calibration results for your records.
Normalization calibration
(TaqMan
®
Array Card only)
During the normalization calibration, the instrument collects and stores the data from
the normalization standards.
IMPORTANT! Normalization calibration is only required for the T
aqMan
®
Array
Card block. Normalization calibration is not required for the 96-well plate blocks or
the 384-well plate block.
For the TaqMan
®
Array Card block, perform a normalization calibration once ev
ery
6 months.
During the normalization calibration, the software captures a series of images of each
normalization array card using each instrument lter. The normalization calibration
yields a “Pass” or “Fail” result for each normalization consumable used.
IMPORTANT! W
ear powder-free gloves and safety glasses when you prepare the
array cards.
For detailed instructions to prepare the calibration array card, see “Preparing array
cards for instrument calibration“ on page 37.
Normalization calibration is only required for the TaqMan
®
Array Card block.
1.
In the QuantStudio
™
Real-Time PCR Software Home tab, click Instrument
Console.
2.
In the Instrument Console, select the icon for your instrument, then click
Manage Instrument.
Note: If the Manage Instrument buon is inactive, add your system to the My
Instruments group.
3.
In the Instrument Manager, start the calibration:
a.
Click
Maintenance, then click Normalization.
b.
In the Normalization Calibration screen, click Start Calibration.
4.
In the Normalization Calibration screen, select the reporter and passiv
e dye
combination that you want to calibrate, then perform the calibration as
instructed.
When to perform
the c
alibration
About the
calibration data
Preparing the
calibration array
card
Perform the
normalization
c
alibration
Chapter 2 Calibr
ate the instrument
Normalization calibration (TaqMan
®
Array Card only)
2
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
59

Applied Biosystems QuantStudio 6 Flex Specifications

General

Brand	Applied Biosystems
Model	QuantStudio 6 Flex
Category	Laboratory Equipment
Language	English