Applied Biosystems QuantStudio 6 Flex - Page 66

3.
Using a permanent marker, mark the side of the empty array card with RNase P.
Mark
here
4.
T
ransfer 100 µL of each solution into the appropriate port of the array card:
For each transfer:
a.
Place the array card on a lab bench, with the foil side down.
b.
Load 100 µL of uid into a pipee.
c.
Hold the pipee in an angled position (∼45 degrees) and place the tip into
the ll port. The ll port is the larger of the tw
o holes on the left side of the
ll reservoir.
GR2158
Microfluidic Card
Card Ports detail
21
1
Fill port
2
Vent port
Chapter 2 Calibrate the instrument
Verify the instrument performance
2
66
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide