b.
Load the calibration plate or array card into the plate holder so that:
• Well A1 of the plate or array card is in the top-left corner of the plate
adapter.
• The barcode faces the front of the instrument.
IMPORTANT! Plates or array cards should be loaded and unloaded by
operators who hav
e been warned of the moving parts hazard and have been
adequately trained.
c.
From the instrument touchscreen, tap to close the instrument tray.
7.
After loading the plate or array card, start the calibration:
a.
In the Setup tab, select Check the box when the ROI calibration plate has
been loaded, then click Next.
b.
In the Run screen, click Start Run.
IMPORTANT! Do not aempt to open the access door during the run. The door
is locked while the instrument is in operation.
Note: Before starting the calibration, the instrument may pause (up to
10 minutes) to allow the heated cov
er to reach the required temperature.
8.
When the run is complete and the Analysis screen displays, conrm the status of
the calibration. The analysis status indicates the success of the calibration, where
passed indicates that the run produced viable calibration data, and failed indicates
that the run did not produce data or that the data it collected is unusable.
Analysis status Action
Passed Click Ne
xt.
Failed
Troubleshoot the failed calibration (see
“Troubleshooting“).
9.
Select the QC tab to review the quality check summary.
Note: The QC tab can provide information on the cause of a failed calibration.
Chapter 2 Calibrate the instrument
Uniformity calibration
2
52
QuantStudio
™
6 and 7 Flex Real-Time PCR Systems v1.6 Maintenance and Administration Guide
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