Chapter 4: Instrument QC 67
3 (Optional) Select Walkaway Operation to run multiple assays automatically.
When the checkbox is deselected, you must click Next Assay at the
Summary view to advance to the next tube.
4 (Optional) Select Automatic Clean to automatically run a 3-minute clean
cycle when you quit the software.
Make sure to install cleaning tubes as shown in Table 4-1. Each tube runs
for 1.5 minutes.
5 Click Save.
6 Gently vortex each tube and place it in its assigned rack position (Table 4-1).
For optimization samples, see page 79.
For accurate results, place each tube only in its assigned rack position. If
you are not running an assay, leave the corresponding position empty.
Table 4-1 Tube positions in the Loader rack
Position Tube
1 unlabeled beads
2 mixed beads for LW and LNW
not containing PerCP-Cy5.5
3 mixed beads for LW
PerCP-Cy5.5 and LNW
PerCP-Cy5.5
4 BD HLA-B27 beads
5–38 optimization samples
39 BD FACS™ Clean solution or
10% bleach solution (3 mL)
40 DI water (3 mL)
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