12. Sampling ABL800 FLEX Operator's Manual
Preparation prior to arterial/venous sampling, Continued
Arterial
sampling
guidelines
The following are guidelines for sampling by arterial puncture:
Sampling method Guidelines
Puncture
• Be careful not to mix venous blood with the arterial
sample. This can occur if a vein is punctured before
locating the artery. Arterial blood (due to the higher
pressure) will generally fill faster than venous blood,
and is lighter in color.
• It is important to expel any air bubbles immediately
without prior agitation. An air bubble with a relative
volume of 0.5 % or more of the blood volume in the
syringe is a potential source of significant error [2, 3].
The effect of such an air bubble increases with the
storage time and agitation of the sample.
• Mix sample thoroughly with heparin immediately
after sampling by inverting the sampler several times
and then rolling the sample between your palms.
A-line
• If aspirating the sample, do so slowly in order to
avoid hemolyzation of the sample
• Expel any air bubbles immediately (see above)
• Mix the sample thoroughly with heparin immediately
after sampling by inverting the sampler several times
and then rolling the sample between your palms.
WARNING – Risk of infection
Disposal of used
sampling devices
Discard the waste as infectious waste [4].
12-6
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