12. Sampling ABL800 FLEX Operator's Manual
Storage and preparation prior to analysis, Continued
Pay attention to
the following:
• For some samples the recommendation above does not apply and individual
guidelines should be used or developed. Examples of these samples are samples
with an increased leukocyte or platelet count, fetal scalp samples, samples with
atypical metabolism, fast-clotting samples, etc. [9, 14].
• Typical metabolic activity in blood samples causes an increase in the lactate
concentration and a decrease in the glucose concentration. For samples within
the typical reference range this corresponds to an average change in the lactate
concentration of 0.25 mmol/L and of –0.2 mmol/L for glucose over 30 minutes
at room temperature [15].
Arterial puncture and A-line samples:
Preparation
prior to analysis
• If there is an air bubble in a sample that has been stored, pH and blood gas
values should not be reported
• Thoroughly mix the sample by inverting the sampler several times and rolling it
between the palms of your hands.
Capillary samples:
• Thoroughly mix the sample with heparin using a mixing wire and magnet.
Capillary blood
sample
measurements
In order to perform in accordance with the analyzer performance specifications,
Radiometer highly recommends to follow the recommendations below.
Capillary blood sample
measurements:
•
When performing capillary blood sample
measurements, use the mixing wire in order to
ensure appropriate mixing of the sample and
thereby prevent clots from being introduced into
the analyzer. The sample should be mixed
immediately after collection, moving the mixing
wire with a magnet 20 times along the full length of
the capillary. This procedure should be repeated
immediately before analyzing the sample.
•
When performing capillary blood sample
measurements and clots are suspected, use a clot
catcher in order to prevent blockage of the
measuring chamber.
•
Do not remove the mixing wire before the sample
is aspirated for measurement. Slide the mixing wire
to the end of the capillary opposite to that from
which the blood is to be aspirated.
Remove both capillary end caps.
Continued on next page
12-10
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