12. Sampling ABL800 FLEX Operator's Manual
Sampling procedures
NOTICE:
This section describes non-Radiometer sampling devices. For information on use
and handling Radiometer sampling devices, please refer to the inserts supplied
with the sampling devices.
Sampling with a
standard syringe
Prepare the following together with a standard syringe (dead space 2-6 %):
• Anticoagulant: lithium or sodium heparin (1000 IU per mL sample)
• Two needles, 20-25 gauge
• Tourniquet − for venous samples
• Skin antiseptic, e.g. 70 % alcohol
• Sterile gauze sponge or cotton wool
• Rubber stopper or syringe end cap (stainless steel)
• Adhesive tape and pressure dressing
Step Action
1.
Mount a needle on the syringe and draw the heparin into the syringe.
2.
Change the needle and flush the barrel walls, then expel air and
heparin through the needle.
3.
Extend the patient’s arm. Apply the tourniquet for a venous puncture.
4.
Locate the blood vessel with the finger tip and clean the skin area with
antiseptic and a sterile gauze sponge or cotton wool.
5.
Insert the needle in the vessel with the bevel upward. Withdraw the
syringe plunger gently to collect blood.
6.
Release the tourniquet (if used), remove the needle and immediately
apply dry gauze or cotton wool over the puncture site. Maintain
pressure on an arterial puncture for at least 5 minutes.
7.
Apply a pressure dressing over the puncture site and elevate the site
for at least 2-3 minutes, if possible.
8.
Expel any air bubbles from the syringe and remove the needle
according to your standard procedure.
9.
Mix the sample by inverting the syringe several times and then rolling
it between your palms.
10
Mix the sample immediately before analysis. Analyze the sample
immediately.
Continued on next page
12-12
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