ABL800 FLEX Operator's Manual 12. Sampling
Sampling procedures, Continued
Sampling with a
test tube
Prepare the following items:
• A test tube and cap
• Anticoagulant: lithium or sodium heparin (1000 IU per mL sample)
• Pipette or syringe and a needle for heparin dosage
• One hypodermic needle, 20-25 gauge
• Tourniquet
• Skin antiseptic, e.g. 70 % alcohol
• Sterile gauze sponge or cotton wool
• Adhesive tape and pressure dressing
Step Action
1.
Transfer the heparin to the test tube using a syringe or a pipette: 50 μL
heparin per 5 mL blood.
2.
Extend the patient’s arm. Apply the tourniquet for a venous puncture.
3.
Locate the vein with the fingertip. Clean the skin area with antiseptic
and a sterile gauze sponge or cotton wool.
4.
Collect the sample according to the procedure in your institution.
5.
Apply dry gauze or cotton wool over the puncture site.
6.
Close the test tube with a cap, then mix the contents by inverting it or
rolling it between your palms about 20 times.
7.
Apply a pressure dressing over the puncture site and elevate the site
for at least 2-3 minutes, if possible.
8.
Mix the sample immediately before analysis. Analyze the sample
immediately.
Pleural liquid
sampling
To collect a pleural liquid sample, do the following:
Step Action
1.
Collect the sample directly from the patient or from a drain.
NOTICE: Radiometer recommends always to use heparinized
samplers for pleural liquids to avoid introducing clots in the analyzer.
Continued on next page
12-13
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