12. Sampling ABL800 FLEX Operator's Manual
Causes of errors in preanalytical phase
Blood sampling should be performed by authorized personnel only. Always handle
blood and the collection devices with care. Avoid direct contact with the sample by
using certified rubber gloves. Sterile techniques are required at all times to avoid
infection.
WARNING –
Risk of infection
Introduction
The major source of errors in blood sample analysis is the preanalytical phase, i.e.
sample collection and handling prior to transfer into the analyzer. In order to avoid
errors, it is necessary to observe certain guidelines during:
• preparation prior to sampling
• sampling
• transport
• storage
• preparation prior to transfer of the sample into the analyzer
Causes of error
The possible causes of errors during the preanalytical phase and preventative
measures are given below.
Cause of error Major affected
parameters
Preventative measures
Air bubbles in
sample
pO
2
* Remove air bubbles immediately.
Clot in sample** pH
• Use correct pre-analytical
handling
• Use Cleaning additive
• Capillaries: Use the mixing
wire
• Use clot catcher.
Sedimented samples ctHb, pO
2,
pCO
2
Mix sample prior to transfer into
analyzer.
Hemolysis cK
+
, cCa
2+
, cCl
–
,
cNa
+
•
Sampling device construction,
narrow needle diameter causes
hemolysis − choose a proper
device for sampling.
•
Avoid squeezing the tissue
during sample collection into a
capillary
•
Avoid mixing too vigorously
See section Storage and
preparation prior to analysis
further in this chapter
•
Continued on next page
12-2
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