3 Prepare the e2695 Separations Module for Operation
106 July 29, 2013, 715003794 Rev. B
• The Pressure field displays the current vacuum level in psia, bar, or
kPa.
Prime the plunger seal-wash pump
The seal-wash solvent lubricates each plunger and flushes away any solvent
or dried salts forced past the plunger seal from the high-pressure side of the
piston chamber. This wash cycle extends the life of the seals. Position the
plunger seal-wash reservoir in a visible location above the solvent
management system, and refill the reservoir as necessary.
For reversed-phase HPLC applications, use an aqueous plunger seal-wash
solution with enough organic content to inhibit bacterial growth. For example,
use an 80% water/20% methanol solution or an 80% water/20% acetonitrile
solution, depending on your application. For GPC seal wash solvent
recommendations, see “GPC solvents and seal choices” on page 293.
To prime the plunger seal-wash pump for first-time use or when the entire
line is dry:
Tip: If you are adding the same plunger seal wash-solution or changing to a
solution that is totally miscible with the existing solution, perform only step 1,
step 5, step 6, and step 8.
1. Press the Diag screen key in the Main screen.
Result: The Diagnostics screen appears. See “Perform main diagnostic
tests” on page 221 for more information on the Diagnostics screen.
2. Attach the syringe adapter to the syringe (both are in the startup kit).
3. Remove the inlet filter from the seal-wash inlet tube.
4. Fill the syringe with the seal-wash solution, and then attach the syringe
adapter to the end of the seal-wash inlet tube.
5. Press the Prime Seal-Wash screen key, and then press the Start screen
key.
Caution: Use separate solutions and containers for the plunger-seal
wash and needle wash. Because the functions of these solutions differ,
using one solution for both functions can compromise the effectiveness of
either needle washing or plunger-seal washing.
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