3 Prepare the e2695 Separations Module for Operation
110 July 29, 2013, 715003794 Rev. B
The following table lists some needle-wash solvents recommended for use with
certain mobile phase conditions. High sample concentrations can require
other needle-wash solvents.
To prime the needle-wash pump:
1. Be sure the needle-wash tubing is properly installed (see “Install
needle-wash and plunger seal-wash reservoirs” on page 61).
2. Press the Diag screen key in the Main screen.
3. In the Diagnostics screen, press the Prime NdlWash screen key.
Tip: The 30-second needle-wash procedure begins. If solvent does not
flow out the waste tube within 30 seconds, press the Start Again screen
key.
Adjust the seal pack
Adjust the seal pack on these occasions:
• You start up the separations module for the first time. (The separations
module does not perform injections or compression checks until the seal
pack has been adjusted.)
Caution: Use separate solutions and containers for needle wash and
plunger seal wash. The solutions’ functions differ, so using one solution
for both functions can compromise the effectiveness of needle or
plunger-seal washing.
Suggested needle-wash solvents:
Chromatographic condition Needle-wash solvent
Buffered aqueous, reversed-phase
80% organic/20% aqueous
1
1. If the aqueous portion of the analytical solution is pH-adjusted to ensure sample solubility,
match the pH of the aqueous portion of the needle-wash solution to it. If acids are used, avoid
long term exposure of the needle-wash system to halide-containing acids.
Nonaqueous, reversed-phase 100% methanol
Normal phase Mobile phase
GPC Mobile phase
Ion exchange Water
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