Wavelength selection
July 29, 2013, 715003794 Rev. B 303
• Common mixed mobile phases
• Chromophores
UV cutoffs for common solvents
The following table shows the UV cutoff (the wavelength at which the
absorbance of the solvent is equal to 1 AU) for some common chromatographic
solvents. Operating at a wavelength near or below the cutoff increases
baseline noise due to the absorbance of the solvent.
UV cutoff wavelengths for common chromatographic solvents:
Solvent
UV Cutoff
(nm)
Solvent
UV Cutoff
(nm)
1-Nitropropane 380 Ethylene glycol 210
2-Butoxyethanol 220 Isooctane 215
Acetone 330 Isopropanol 205
Acetonitrile 190 Isopropyl chloride 225
Amyl alcohol 210 Isopropyl ether 220
Amyl chloride 225 Methanol 205
Benzene 280 Methyl acetate 260
Carbon disulfide 380 Methyl ethyl ketone 330
Carbon tetrachloride 265 Methyl isobutyl
ketone
334
Chloroform 245 Methylene chloride 233
Cyclohexane 200 n-Pentane 190
Cyclopentane 200 n-Propanol 210
Diethyl amine 275 n-Propyl chloride 225
Dioxane 215 Nitromethane 380
Ethanol 210 Petroleum ether 210
Ethyl acetate 256 Pyridine 330
Ethyl ether 220 Tetrahydrofuran 230
Ethyl sulfide 290 Toluene 285
Ethylene dichloride 230 Xylene 290
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