D Solvent Considerations
294 July 29, 2013, 715003794 Rev. B
To prevent premature plunger seal failure when using toluene,
o-dichlorobenzene (o-DCB), or 1,2,4 trichlorobenzene (TCB), Waters
recommends the use of the optional Alliance Clear Seals, the removal of
installed seal wash seals, and no seal wash solvent. The use of graphite-filled
PTFE (black) seals is not recommended for GPC applications with the e2695
Separations Module.
Solvent compatibility
The separations module is constructed of high-quality (316) stainless steel
components that, with some minor restrictions, can be used with all solvents.
Dimethylacetamide
(DMAC)
c
Do not use Strongly
recommended
100% methanol
Hexafluoroisopro-
panol (HFIP)
d
Recommended Strongly
recommended
50:50
methanol/water
Methylene chloride Recommended Recommended 100% methanol
N,N-dimethylform-
amide (DMF)
c
Do not use Strongly
recommended
50:50
methanol/water
N-methyl-2-pyrroli-
done (NMP)
c
Do not use Strongly
recommended
50:50
methanol/water
Tetrahydrofuran
(THF)
Strongly
recommended
Strongly
recommended
50:50
methanol/water
Water
e
Recommended Recommended 20:80
methanol/water
a. Package contains 2 seals, P/N: WAT270938.
b. Package contains 2 seals, P/N: 700001326.
c. This solvent is typically used with an additive of 0.05M lithium chloride or lithium bromide.
d. HFIP is typically used with an additive of 0.05M sodium trifluoroactetate.
e. Most aqueous GPC separations use a salt or buffer.
Recommended plunger seal and seal wash solution to be used for GPC
solvents: (Continued)
GPC Solvent
With standard
seal
a
Fluoroloy G
(yellow)
With optional
seal
b
Alliance Clear
Seal
(opaque)
Seal wash
solution
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