Wavelength selection
July 29, 2013, 715003794 Rev. B 305
Refractive indices of common solvents
The following table lists the refractive indices for some common
chromatographic solvents. Use this table to verify that the solvent you intend
to use for your analysis has a refractive index (RI) significantly different from
the RIs of the sample components.
Refractive indices for common chromatographic solvents:
Solvent RI Solvent RI
Fluoroalkanes 1.25 Tetrahydrofuran (THF) 1.408
Hexafluoroisopropanol
(HFIP)
1.2752 Amyl alcohol 1.410
Methanol 1.329 Diisobutylene 1.411
Water 1.33 n-Decane 1.412
Acetonitrile 1.344 Amyl chloride 1.413
Ethyl ether 1.353 Dioxane 1.422
n-Pentane 1.358 Ethyl bromide 1.424
Acetone 1.359 Methylene chloride 1.424
Ethanol 1.361 Cyclohexane 1.427
Methyl acetate 1.362 Ethylene glycol 1.427
Isopropyl ether 1.368 N,N-Dimethyl Formamide
(DMF)
1.428
Ethyl acetate 1.370 N,N-Dimethyl Acetamide
(DMAC)
1.438
1-Pentene 1.371 Ethyl sulfide 1.442
Acetic acid 1.372 Chloroform 1.443
Isopropyl chloride 1.378 Ethylene dichloride 1.445
Isopropanol 1.38 Carbon tetrachloride 1.466
n-Propanol 1.38 Dimethyl sulfoxide
(DMSO)
1.477
Methylethylketone 1.381 Toluene 1.496
Diethyl amine 1.387 Xylene ~1.50
n-Propyl chloride 1.389 Benzene 1.501
Methylisobutylketone 1.394 Pyridine 1.510
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