4 Front Panel Control
132 July 29, 2013, 715003794 Rev. B
Purge the 2410 and 410 reference cell
You can purge the reference and sample sides of the Waters 2414, 2410, and
410 Differential Refractometers using the Purge 410 Reference direct
function. Purge the flow path whenever you change solvents or you experience
an unexpected loss in sensitivity due to excess noise or drift.
To purge the 2414, 2410, or 410 reference cell:
1. In the Status screen, enter the flow rate and composition at which you
want to purge the 2414, 2410, or 410 reference cell.
2. Press the Direct Function screen key.
3. Select Purge 410 Reference, and then press Enter.
4. In the Purge 410 Reference dialog box, specify the amount of time that
you want to pump fresh solvent through the reference cell, and then
press OK.
5. Press the OK screen key.
Equilibrate the system
To equilibrate the HPLC system, the separations module pumps solvent at the
initial conditions specified in the Status screen or in the separation method
(see Chapter 6), flushing the flow path of any sample and solvent run at other
compositions and preparing the system for the next run.
To equilibrate the system:
1. Press Menu/Status to display the Status screen.
2. In the Status screen, specify initial conditions, or, select the separation
method that contains the initial conditions at which you want to
equilibrate.
3. Press the Direct Function screen key.
4. Select Equilibrate, and then press Enter.
5. In the Equilibration dialog box, specify a length of time for equilibrating
the system, and press the OK screen key.
Result: The separations module begins the equilibration procedure.
When the system is equilibrated, the separations module enters the idle
state. The flow rate remains at the value specified in the Status screen.
To Next Page
Loading...
